ytkK [2018-02-13T02:35:59.000Z]

similar to 3-oxoacyl- acyl-carrier protein reductase

locus: BSU29420

pI: 7.09

mw: 27.82 kDa

protein length: 255 aa

gene length: 765 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,010,661 → 3,011,428

The protein

Paralogous protein(s)

• [protein|439B468A13137000FB42E9389391CB4986FFED84|FabG] (32,1%)

Structure

• [PDB|3I4F] (from Bacillus Thuringiensis 69% identity)

Expression and Regulation

Operons

• genes: [gene|3BF3EC6B3139744D968B2FF18CBEEEF15B591AFF|ytkK]-[gene|8B28AA1A88FA56C9F4742F668B28F3323715D948|ytkL]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-A158 (ytkK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/158 NBRP B. subtilis, Japan]
• BKE29420 (Δ[gene|3BF3EC6B3139744D968B2FF18CBEEEF15B591AFF|ytkK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATGAATACCTCCCAAAG, downstream forward: _UP4_TAAGTTTTTCAGCTTTTTAA
• BKK29420 (Δ[gene|3BF3EC6B3139744D968B2FF18CBEEEF15B591AFF|ytkK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATGAATACCTCCCAAAG, downstream forward: _UP4_TAAGTTTTTCAGCTTTTTAA